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Genetic diversity analysis of rice varieties (Oryza sativa L.) based on morphological, pedigree and DNA polymorphism data

Published online by Cambridge University Press:  12 February 2007

Jorge Luis Fuentes ,
Maria Teresa Cornide ,
Alba Alvarez ,
Enrique Suarez  and
Ernesto Borges
Jorge Luis Fuentes*
Affiliation:
Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Apartado Postal 6122, calle 30, #502. e/ 5ta y 7ma, Miramar, Playa, C. Habana, Cuba
Maria Teresa Cornide
Affiliation:
Instituto de Investigaciones de la Caña de Azúcar (INICA), Carretera CAI Martínez Prieto, Marianao, Ciudad de la Habana, Cuba
Alba Alvarez
Affiliation:
Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Apartado Postal 6122, calle 30, #502. e/ 5ta y 7ma, Miramar, Playa, C. Habana, Cuba
Enrique Suarez
Affiliation:
Instituto de Investigaciones del Arroz (IIA), Bauta, La Habana, Cuba
Ernesto Borges
Affiliation:
Instituto de Investigaciones del Arroz (IIA), Bauta, La Habana, Cuba
*
*Corresponding author: E-mail: fuentes@ceaden.edu.cu
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Abstract

The diversity within 20 rice varieties used as progenitors in Cuban rice breeding programmes was analysed with respect to agro-morphological traits, pedigree and DNA markers. Eleven agro-morphological traits were scored, and phenotypic (Euclidian) distances between the rice varieties were calculated. Sixty random amplified polymorphism DNA (RAPD) and 115 amplified fragment length polymorphism (AFLP) bands served to determine Dice's distance estimates. Cluster analyses were performed based on genetic distance matrices using the unweighted pair-group method of arithmetical means (UPGMA) as the clustering method. This analysis showed five phenotypic, six genealogical, five RAPD and six AFLP diversity groups. Genetic diversity estimates based on RAPD data, but not on AFLP, efficiently represented the genetic parentage and phenotypic diversity between rice varieties. Combined diversity estimates allowed the identification of 11 different genetic pools and permitted a more effective separation of the progenitor set than those obtained solely by phenotypic and genealogical information. The results of this study stress the necessity to diversify rice parental stocks for further breeding purposes.

Keywords

AFLPgenealogyphenotypic descriptorsRAPD
Type
Research Article
Information
Plant Genetic Resources , Volume 3 , Issue 3 , December 2005 , pp. 353 - 359
Copyright
Copyright © NIAB 2005

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